Journal: Scientific Reports

Article Title: Irisin activates the AMPK-Beclin1 signaling pathway to regulate pulmonary autophagy induced by CSE + LPS

doi: 10.1038/s41598-025-31824-2

Figure Lengend Snippet: Effects of Different Intervention Conditions on Pulmonary Histopathology, Irisin Expression, and Inflammatory Response in Mice. ( A, C, D ) H&E staining of pulmonary tissue (100× magnification, scale bar = 200 μm) ( n = 5). Morphometric measurements of Mean Linear Intercept (MLI) (µm) ( n = 5). Lung injury scores were assessed from histological analyses of mouse lung tissues ( n = 5). ( B, E ) Immunofuorescence of FNDC5 (irisin) in lung tissues. (400× magnification, scale bar = 50 μm) ( n = 5). ( F–H ) The levels of IL-1β, IL-6, and TNF-α in bronchoalveolar lavage fluid (BALF) from mice ( n = 5). Data are presented as mean ± SD. Signifcant differences were presented as * P < 0.05, ** P < 0.01, *** P < 0.001,**** P < 0.0001. ns indicates no statistical significance.

Article Snippet: Cell Signaling Technology (Danvers, MA, USA) provided AMPK(1:1000, 5831T), p-AMPK(1:1000, 2535T), ATG5(1:1000, 12994T); LC3(1:1000, PM036), P62(1:1000, PM045) and Beclin1(1:1000, PD017) were obtained from MBL(Beijing China); FNDC5(1:200, bs-8486R) was obtained from Bioss (Beijing China); Lipofectamine 2000 was purchased from Thermo Fisher Scientific (MA, USA); ATG5 siRNA/control siRNA was purchased from (Genepharma, Suzhou, China); Compound C were obtained from AmBeed (Arlington Hts, IL USA); The reagents required for cell culture, including RPMI 1640, Fetal Bovine Serum (FBS), penicillin–streptomycin (100×), Phosphate Buffered Saline (PBS) were purchased from Gibco or Solarbio; TNF-α Elisa Kit, IL-6 Elisa Kit, and IL-1β Elisa Kit were purchased from Multi Sciences (China); Ad-mCherry-GFP-LC3B were obtained from Beyotime (C3011, Nantong, China); Irisin was purchased from Cayman Chemical Company.

Techniques: Histopathology, Expressing, Staining

Journal: Frontiers in Aging Neuroscience

Article Title: Aerobic exercise and gut flora: a key link to improved cognitive impairment in mice with Parkinson’s disease

doi: 10.3389/fnagi.2025.1630003

Figure Lengend Snippet: SNpc neuron damage in experimental animals in the first stage. (A,B) Midbrain substantia nigra immunofluorescence staining, blue fluorescence for DAPI, green fluorescence for TH + cells. (C–F) WB midbrain TH, FNDC5, BDNF, β-actin strip and statistical map. (G–I) Hippocampal dendrite spine density and statistical map. n = 3, ** p < 0.01.

Article Snippet: The following antibodies were used: anti-TH antibody (Cat#SAB2701683, Sigma, USA), anti-ZO-1 antibody (Cat#13663, CST, USA), anti-Occludin antibody (Cat#ab222691, Abcam, UK), anti-cluadin-5 antibody (Cat#ab172968, Abcam, UK), anti-BDNF antibody (Cat# 28205-1-AP, Proteintech, USA), anti-FNDC5 antibody (Cat# 82671-1-RR, Proteintech, USA), anti-cAMP antibody (Cat# 82940-1-RR, Proteintech, USA) anti-PGC1- α (Cat# HY- P80783 , MedChemExpress, USA) antibody, anti-CREB antibody (Cat# HY- P80092 , MedChemExpress, USA), anti- β -actin antibody (Cat# 60008-1-Ig, Proteintech, USA), Anti-GAPDH Antibody (Cat# 10494-1-AP, Proteintech, USA), Goat Anti-Rabbit Antibody (Cat# VSA27, Vicmed, XZ, CHN), Goat Anti-Mouse Antibody (Cat# VSA32, Vicmed, XZ, CHN).

Techniques: Immunofluorescence, Staining, Fluorescence, Stripping Membranes

Journal: Frontiers in Aging Neuroscience

Article Title: Aerobic exercise and gut flora: a key link to improved cognitive impairment in mice with Parkinson’s disease

doi: 10.3389/fnagi.2025.1630003

Figure Lengend Snippet: Bands of FNDC5, BDNF, cAMP, PGC1-α, CREB, and β-actin in the midbrain of WB from A to F statistical map, n = 3. (G,H) Clustering heat map of major plasma metabolites. (I,J) Heat map of correlation between major intestinal differential bacteria and major plasma metabolites, n = 6. Pos: anode, Neg: cathode, Parkinson’s disease mice in group B (MPTP group), aerobic exercise mice in group C (MPTP+Ex group), * p < 0.05, ** p < 0.01. This schematic illustrates the pathway through which aerobic exercise mediates cognitive rehabilitation in Parkinson’s disease, based on our investigational results.

Article Snippet: The following antibodies were used: anti-TH antibody (Cat#SAB2701683, Sigma, USA), anti-ZO-1 antibody (Cat#13663, CST, USA), anti-Occludin antibody (Cat#ab222691, Abcam, UK), anti-cluadin-5 antibody (Cat#ab172968, Abcam, UK), anti-BDNF antibody (Cat# 28205-1-AP, Proteintech, USA), anti-FNDC5 antibody (Cat# 82671-1-RR, Proteintech, USA), anti-cAMP antibody (Cat# 82940-1-RR, Proteintech, USA) anti-PGC1- α (Cat# HY- P80783 , MedChemExpress, USA) antibody, anti-CREB antibody (Cat# HY- P80092 , MedChemExpress, USA), anti- β -actin antibody (Cat# 60008-1-Ig, Proteintech, USA), Anti-GAPDH Antibody (Cat# 10494-1-AP, Proteintech, USA), Goat Anti-Rabbit Antibody (Cat# VSA27, Vicmed, XZ, CHN), Goat Anti-Mouse Antibody (Cat# VSA32, Vicmed, XZ, CHN).

Techniques: Clinical Proteomics, Bacteria